Fig. 2
From: Human placenta mesenchymal stem cell-derived exosomes delay H2O2-induced aging in mouse cholangioids
Features of long-term cultured cholangioids. a Organoids (P1 day 12) observed by light microscopy. Cells scattered inside and outside the organoid lumen (top, right bottom); shrunken organoids (left bottom) (scale bar, 100 μm). b CK7-positive cholangiocytes within organoids (P0 day 11). Cells remained close together and developed into more than one layer, as observed by confocal laser scanning microscopy (scale bar: left, 50 μm; right, 100 μm). c SA-β-gal staining of cholangioids (P1 day 12), some senescent cholangiocytes (green, top) and completely senescent cholangioids (bottom) (scale bar, 100 μm). d Viability/cytotoxicity assay of organoids (P1 day 4). Dead cells (red) aggregated inside the organoid lumen (green) (scale bar, 50 μm). e Organoids (P1 day 2) observed by light microscopy. White arrow indicates single cholangiocyte, and black arrow indicates organoids grown from single cholangiocytes (scale bar, 200 μm)