Fig. 5
From: Human placenta mesenchymal stem cell-derived exosomes delay H2O2-induced aging in mouse cholangioids
hPMSC-derived exosomes have a protective effect in senescent cholangioids. a Comparison of organoids among the Ctrl, Sen, and Exo groups at 0 h (top), 48 h (middle), and 120 h (bottom). Typical senescent organoids at 120 h are indicated by white circles (scale bar, 200 μm). b Comparison of the immunohistochemical staining results for proliferation marker Ki67, as well as the senescence-associated markers p16INK4a and p21WAF1/Cip1 among the Ctrl, Sen, and Exo groups at 120 h (× 40 objective, scale bar, 200 μm; rectangle indicates × 80 objective). c–e Semi-quantitative analysis of p16INK4a, p21WAF1/Cip1, and Ki67 immunohistochemical staining results at 120 h. These data were acquired with a × 20 objective, and Image-Pro Plus software was used to analyze 10 randomly selected areas (two-tailed t test, mean ± standard error of the mean, n = 10, ***P < 0.001, **P < 0.01, *P < 0.05). f Percentages of SA-β-gal-positive organoids in 30 randomly selected areas, counted under a × 20 objective with bright field illumination (two-tailed t test, mean ± SD, n = 30, ***P < 0.001, **P < 0.01). g Representative CK19-positive cholangioids (cyan) in 3D form. The area of p21WAF1/Cip1-positive staining was greater in the Sen group than in the other two groups, while the Exo group showed the smallest area (scale bar, 100 μm). h SA-β-gal-positive cholangioids. Organoids were collected and analyzed at 120 h (green, scale bar, 50 μm)