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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Process development and safety evaluation of ABCB5+ limbal stem cells as advanced-therapy medicinal product to treat limbal stem cell deficiency

Fig. 4

Effect of fibrin gel on ABCB5+ limbal stem cells (LSCs). a Fibrin gel is used as carrier for transplantation of ABCB5+ LSCs onto the debrided cornea. b Calcein staining demonstrates viability of ABCB5+ LSCs after 72 h of culture in fibrin gel. c Fibrin gel does not create hypoxic conditions capable of stimulating VEGF secretion by ABCB5+ LSCs in vitro as shown by VEGF secretion after cultivation under hypoxic conditions (as compared to donor-matched ABCB5 limbal cells, left) and in fibrin gel (right). This experiment was performed on LSCs from three donors. Please note different y-axes scales between hypoxia and fibrin gel

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