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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: miR-133a silencing rescues glucocorticoid-induced bone loss by regulating the MAPK/ERK signaling pathway

Fig. 3

Silencing miR-133a promoted osteoblast differentiation in GC-treated MSCs. a Western blotting analysis for osteogenesis- and MAPK/ERK signaling-related proteins in MSCs after different treatments. b, c Gray analysis for the western blotting results of ALP and Runx2. d, e qRT PCR analysis of ALP and Runx2 mRNA in MSCs. f Representative images of alizarin red staining in MSCs after different treatments (× 100 magnification). g Quantification of alizarin red staining. hj Gray analysis for the western blotting results of ERK1/2, p-ERK1/2, and FGFR1 proteins with osteogenesis induction medium. k qRT PCR analysis of FGFR1 mRNA in MSCs with osteogenesis induction medium (each condition was performed in triplicate, * P < 0.05 relative to the DEX group)

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