Fig. 4

Silencing miR-133a inhibited adipocyte differentiation in GC-treated MSCs. a Western blotting analysis for adipogenesis- and MAPK/ERK signaling-related proteins in MSCs after different treatments. b, c Gray analysis for the western blotting results of aP2 and PPARγ. d, e qRT PCR analysis of aP2 and PPARγ mRNA in MSCs. f Representative images of oil red staining in MSCs after different treatments (×400 magnification). g Quantification of oil red staining. h–j Gray analysis for the western blotting results of ERK1/2, p-ERK1/2, and FGFR1 proteins in MSCs with adipogenesis induction medium. k qRT PCR analysis of FGFR1 mRNA in MSCs with adipogenesis induction medium (each condition was performed in triplicate, * P < 0.05 relative to the DEX group)