Fig. 7

Silencing miR-133a attenuates marrow fat accumulation and trabecula damage in distal femurs of GIO rats. a Representative images of H&E staining showing trabecula and marrow adipocytes in distal femur of each group. b Quantification of number of adipocytes in distal femur of each group. c, d Representative images of immunofluorescence staining (c) and quantification of perilipin area /total area (T.Ar) in distal femur. e, f Representative images immunohistochemical staining (e, arrows indicate osteocalcin positive osteoblasts) and quantification of osteoblasts per bone surface (BS) in distal femur (scale bar 50 μm, *P < 0.05 relative to the MP group)