Fig. 1

Characterization of hAECs, detection of the survival hAECs encapsulated in SA-BG, and schematic illustration of the surgical procedure. a The morphology of cultured hAECs was observed under a microscope. Scale bar 100 μm. b Flow cytometry analysis of cell surface markers on hAECs. The isotypes (ISO) of SSEA4 and CD324 were used as negative controls. c Immunostaining images showed the high expression of epithelial marker (CK18) and stem cell marker (TRA-1-60). Scale bar 100 μm. d The fabrication method of SA-BG-loaded hAECs and CM. e Representative live/dead images of hAECs encapsulated in SA-BG at days 1, 7, 10, and 14, respectively. hAECs encapsulated in SA-BG treated with 70% alcohol were positive for PI. Live cells were shown green color and dead cells were red color. Scale bar 100 μm. f Bright field image of hAECs capsulated in SA-BG at day 1 (a) and 14 (b). Scale bar 100 μm. g The percentage of live cells to total cells. h Schematic of the experimental procedure for the transplantation of SA-BG-loaded hAECs/CM into mice with chemotherapy-induced POF