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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Sodium alginate-bioglass-encapsulated hAECs restore ovarian function in premature ovarian failure by stimulating angiogenic factor secretion

Fig. 2

Morphological observation and follicle count in ovaries of mice at day 28 after transplantation. a Representative photographs of ovarian sections stained with H&E from different treatment groups. Scale bars 200 μm and 100 μm. b Immunochemical staining of DDX4 in ovarian sections from different groups. The bold black arrowhead indicated DDX4-positive primordial follicles. Scale bars 25 μm. c–f Column displayed the number of calculated primordial follicles, primary follicles, secondary follicles, and antral follicles in the whole ovary. Sections taken into counting were obtained in every fifth section. g Column showed the number of total follicles in the ovary from different treatment groups. h Column displayed the number of DDX4-positive primordial follicles in the ovarian section. Data are presented as means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. Bu/Cy group; #p < 0.05, ##p < 0.01, ####p < 0.0001 vs. SA-BG group; $ p < 0.05 vs. SA-BG/hAECs group

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