Fig. 3

Immunofluorescent staining and detection of related proteins at day 3 after transplantation. a Representative images of proliferative cells (Ki67 positive, green color). Scale bars 50 μm and 25 μm. b Representative images of apoptotic cells (TUNEL positive, red color) in different groups. Scale bars 50 μm and 25 μm. c Column showed the proliferative rate of granulosa cells in the antral follicles. d Column displayed the apoptosis rate of granulosa cells in the antral follicles. Nuclei were counterstained with DAPI (blue). e, f Expression of PCNA protein in different groups. g, h Expression of apoptotic proteins in different groups (n = 3). Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. Bu/Cy group; ^##p < 0.01 vs. SA-BG group; ^$p < 0.05 vs. SA-BG/hAECs group