Skip to main content
Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Use of constitutive and inducible oncogene-containing iPSCs as surrogates for transgenic mice to study breast oncogenesis

Fig. 5

Trifluorescence studies of extirpated tumors in the transgenic IPSC clones. Triple fluorescence studies on PyVT-iPSC generated extirpated tumors demonstrated DAPI blue nuclear autofluorescence (a), GFP green autofluorescence (b), and Alexa Fluor® 594 red immunofluorescence using goat anti-rat added to rat monoclonal to PyVT antigen (c) and its merged overlay (d). Strong cytoplasmic expression of the PyVT antigen is observed. Similarly, triple fluorescence studies on ErbB2-iPSC generated extirpated tumors demonstrated DAPI blue nuclear autofluorescence (e), GFP green autofluorescence (f), and Alexa Fluor® 594 red immunofluorescence using goat anti-rabbit added to rabbit polyclonal antibody to ErbB2 (g) and its merged overlay (h). Strong membrane expression of the ErbB2 antigen is observed

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512