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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Use of constitutive and inducible oncogene-containing iPSCs as surrogates for transgenic mice to study breast oncogenesis

Fig. 7

Digital image analysis with specific recognition algorithms (SRAs) and quantitation of transgene expression. Digital image analysis of relative fluorescence and colorimetric immunocytochemistry of representative TMA cores illustrate quantitative PyVT cytoplasmic immunoreactivity (a) and quantitative ErbB2 membrane immunoreactivity (b). Relative immunocytochemical intensity levels of both transgenes in normal ducts, ducts with hyperplasia, ducts with DCIS, and invasive carcinoma and metastatic carcinoma in the tumors arising in the original transgenics v the tumors arising from the injected iPSC clones are depicted (c). Laser-capture microdissection (d) of tumoral areas subjected to RNA extraction and real-time RT-PCR depicts relative mRNA expression levels in the same groups and tumoral areas (e)

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