Fig. 2

a Adipogenic and osteogenic differentiation analysis of MenSCs and BMMSCs, staining the cells with oil red, and alizarin, respectively. Control cells were incubated using standard complete DMEM medium and stained with the same dyes after differentiation ended. The cells were cultivated in adipogenic/osteogenic medium for 21 days. Scale bar 100 μm. b Quantitative adipogenic and osteogenic differentiation analysis of MenSCs and BMMSCs (21 days), dissolving lipid droplets (stained with oil-red) in isopropanol, and calcium hydroxyapatite crystals (stained with alizarin red) in cetylpyridinium chloride solutions. Absorbance measured at 520 nm and 562 nm, respectively. Data are presented as ratio to control, mean ± SD. Horizontal bars represent p ≤ 0.05