Fig. 8

Atelocollagen/PLLA constructs with MenSCs and BMMSCs, stimulated by combination of activin A (50 ng/mL, first 2 days) and TGF-β3 (10 ng/mL, 21 days) or only with TGF-β3 (10 ng/mL, 21 days) or cultured without growth factors (21 days), followed by a subcutaneous insertion into mice for 9 weeks. a Experimental mouse with 3 cell-loaded constructs implanted subcutaneously; removal of implants at the end of experiment (9 weeks); removed construct. b Histological analysis of constructs after 9 weeks in vivo. Control constructs were differentiated under the same conditions, excluding growth factors. Safranin O staining. × 100 and × 400 magnification