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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Combined topical and systemic administration with human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes markedly promoted cutaneous wound healing and regeneration

Fig. 1

Isolation and characterization of hADSC and hADSC-Exo. a Spindle hADSC were observed through the microscope. Scale bar = 25 μm. b–d Adipogenic, osteogenic, and chondrogenic differentiation of hADSC. Cells differentiated into adipocyte, osteoblasts, and chondrogenic were detected using Oil Red O, Alizarin Red, and Alcian Blue, respectively. Scale bar = 25 μm. e Flow cytometry of hADSC surface markers CD29, CD73, CD105, SSEA-3, SSEA-4, and HLA-DR. f Nanoparticle analysis of ADSC-Exo. g TEM analysis of exosomes. Scale bars = 500 nm. h Immunoblotting for CD9 and CD63 in exosomes

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