Fig. 4

Histological assessment of scar after wound healing in different groups. a Masson staining of wounded skin sections in different groups at day 12 post-wounding. Scale bar = 200 μm. b The results of immunohistochemical analysis of collagen I and collagen III were same as above. Scale bar = 200 μm. c Quantitative analysis of collagen I, collagen III, and the ratio of collagen I/collagen III. d Immunohistochemical analyses were performed by using anti-Filaggrin, anti-Loricrin, and anti-AQP3 antibodies in skin wounds. Scale bar = 200 μm. e Quantification of Filaggrin, Loricrin, and AQP3 IHC stained tissues. The rhEGF was used as a positive control. Results are presented as mean ± standard error of the mean; n = 8 for each group. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 vs vehicle control group