Fig. 7

BM-MSCs enhance wound healing through transdifferentiation into endothelial cells and increasing VEGF expression. a mKate2 positive cells (red) were colocalized with CD31(green) in the wound site and right gastrocnemius tissues at day 14 (RFP and CD31 IHC staining, 40 × 10 or 100 × 10). b Representative images of VEGF in wound site at day 14 (VEGF IHC staining, 40 × 10). c VEGF content in the wound at day 14. d Western blotting analysis of VEGF, p-AKT, AKT, PTEN, and Actin expression in wound site at day 14 among three groups. The quantification of PTEN/Actin (e), p-AKT/AKT (f), and VEGF/Actin (g) by Western blotting. Values are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001