Fig. 3

The effect of ADSC-Exo on the excisional model of BALB/c mice. a Schematic diagram of the experimental processes. b, c Digital photographs of wound area treated with ADSC-Exo or PBS post-wounding on day 3, 5, 7, 10, and 14. Histogram showed the relative distances analyzed by Image-Pro Plus. d The trace of PKH26-labeled ADSC-Exo in the wound of BALB/c mice on day 5, scale bars = 200 μm. e qRT-PCR analysis of Col1 and α-SMA in wound tissues of BALB/c mice treated with PBS or ADSC-Exo, graph represented the expression of Col1 and α-SMA relative to that of GAPDH. f, g Immunoblot analysis of Col1, Col3, and α-SMA in wound tissues of BALB/c mice treated with PBS or ADSC-Exo, histogram showed the relative band density to β-actin. h Representative images of H&E and Masson’s trichrome staining of the sections on day 14 post-wounding in normal skin, wound tissues treated with PBS or ADSC-Exo groups, scale bar = 400 μm, 100 μm. The data was shown as mean ± SEM (*p < 0.05, **p < 0.01)