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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Exosomes derived from human adipose mesenchymal stem cells attenuate hypertrophic scar fibrosis by miR-192-5p/IL-17RA/Smad axis

Fig. 5

The verification of targeted regulatory relationship between miR-192-5p and IL-17RA in HSFs. a The binding sites predicted by bioinformatics algorithms. b, c The targeted modulation measured by luciferase reporter gene assays. d qRT-PCR analysis of miR-192-5p in HSFs transfected with mimics, inhibitors, and their corresponding negative control. e–g The expression of IL-17RA in HSFs transfected with mimics, inhibitors, and their corresponding negative control examined by qRT-PCR and WB. h, i Immunoblotting analysis of the protein expression of IL-17RA, Col1, Col3, and α-SMA in HSFs exposed to miR-192-5p mimics/mimic NC, IL-17RA siRNA/scramble siRNA, graphs showed their levels relative to that of β-actin. Every experiment was repeated at least three times, the data was shown as mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001)

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