Fig. 7

The effect of IL-17RA silence on HSFs and excisional model of BALB/c mice. a The efficiency of IL-17RA siRNA in HSFs examined by immunofluorescence staining, scale bar = 50 μm. b qRT-PCR analysis of IL-17RA, Col1, and α-SMA in HSFs treated with IL-17RA siRNA or scramble siRNA. c Immunoblot analysis of IL-17RA, Col1, Col3, and α-SMA in HSFs exposed to IL-17RA siRNA or scramble siRNA, graphs showed the relative band density to β-actin. d, e The morphology of wound healing observed in different periods. Line chart showed the relative wound areas. f The trace of Lv-IL-17RA in wound tissues on day 5 detected by frozen sections, scale bar = 200 μm. g qRT-PCR analysis of IL-17RA, Col1, and α-SMA in wound tissues of BALB/c mice stimulated with mEGFP-NC or IL-17RA shRNA-EGFP, the graph represented their expression relative to that of GAPDH. h Immunoblot analysis of the aforementioned molecules. i Representative images showed the histopathological change, collagen deposition, and positive α-SMA+ fibroblasts determined by H&E, Masson’s trichrome, and immunohistochemistry staining, respectively, scale bar = 400 μm, 100 μm, 50 μm. The data was shown as mean ± SEM (*p < 0.05; **p < 0.01)