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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Therapeutic potential of small extracellular vesicles derived from lipoma tissue in adipose tissue regeneration—an in vitro and in vivo study

Fig. 1

Characterization of ADSCs, sEV-LT and sEV-AT. a ADSCs exhibited the typical spindle-shaped morphology, as shown in the picture. Scale bar = 200 μm. b Representative photographs of Oil red O staining for adipocytes. Scale bar = 50 μm. c Representative photographs of Alizarin Red S staining for osteoblasts. Scale bar = 50 μm. d Flow cytometry analysis showed that these cells were highly positive for CD105, CD90, and CD73 but negative for CD34 and CD31. Abbreviations: PE, phycoerythrin. e The ultrastructure of sEV-LT and sEV-AT under transmission electron microscopy. Scale bar = 100 nm. f The size distribution profile of sEV-LT and sEV-AT by dynamic light scattering. g The expression of the sEV markers CD9, CD63, and TSG101 was confirmed by immunoblotting

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Stem Cell Research & Therapy

ISSN: 1757-6512