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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Secretomes of human pluripotent stem cell-derived smooth muscle cell progenitors upregulate extracellular matrix metabolism in the lower urinary tract and vagina

Fig. 3

pro-MMP2 and pro-MMP9 activity of pSMC-CM-treated bSMCs (a) and vaginal fibroblasts (b). Zymographic evaluation of MMP activities in concentrated supernatant from bSMCs and vaginal fibroblasts treated with different pSMC-CM. Each bar plot represents 6 separate experiments run on 6 gels to illustrate the statistical trends for each experiment. Each experiment consists of the SMGS-media-only, SMGS, CM at P0, and CM at P3 groups (black, orange, blue, and pink bars). The expression levels for each bar can be compared within each experiment, but not between experiments. Data shown represent the mean ± SD from three independent experiments. Huf5 CM conditioned medium from Huf5 iPSC-derived pSMCs, BIR CM conditioned medium from BIR iPSC-derived pSMCs, SMGS supernatant from bSMC or fibroblasts treated with SMGS only, SMGS media only = concentrated SMGS media only, not the supernatant from cell culture. *Significant difference between groups (p < 0.05)

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Stem Cell Research & Therapy

ISSN: 1757-6512