Fig. 1
Subconjunctival administration of allogeneic MSCs prolongs corneal allograft survival. Female Balb/c mice served as recipients for female C57BL/6 donor corneas. Different doses of allogeneic (donor-derived) MSCs were injected subconjunctivally 7 days before transplantation (D−7). Mice were observed every 2/3 days. a Kaplan-Meier survival curve analysis of allogeneic transplant controls (black line) (n = 9), corneal allograft + 5 × 105 MSCs (pink line) (n = 6), and corneal allograft + 5 × 104 MSCs (green line) (n = 4) (Log-rank (Mantel-Cox) test, **p ≤ 0.01, ***p ≤ 0.001). b Opacity and c neovascularization scores up to POD 40. d Representative light microscopy images of corneal allografts taken at two post-transplantation time points from two separate mice either treated with low-dose allo-MSCs or untreated. n = 4–6 with numbers per treatment group the same as in a. Error bars show mean + SD