Fig. 6

uMSC-EVs inhibited TGF-β1-induced myofibroblast differentiation in vitro. WML2 fibroblast cells were cultured for 48 h with TGF-β1 to induce myofibroblast differentiation, some of the cells were also treated with uMSC-EVs as indicated, then expression levels of α-SMA (a, b), TGF-β2 (c, d), and TGF-βR2 (c, e) were detected in different treatments using qRT-PCR and IF. Scale bar = 200 μm. Mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; n = 3. TGF-β1, transforming growth factor-β1