Fig. 1

Comparison of hBMSC-Luc/GFP persistence in the femurs of mice with and without 2 Gy sub-lethal irradiation prior to transplant. a Representative IVIS images of the bioluminescence signal from mice that had either been non-irradiated or had been conditioned with 2 Gy irradiation. b Graphical display of the bioluminescence data for each animal across the 4-week study. Horizontal bar represents the group average. Linear regression curves were fitted to each data set. The signal in non-irradiated mice was significantly lower than in irradiated animals from week 2 to week 4 (2-way ANOVA with multiple comparison using Holm-Sidak correction). Significance between groups marked with asterisk; ** p ≤ 0.01, *** p ≤ 0.001. Data represents pooled data the two donor hBSMC and each with a biological repeat (total group n = 16 mice not irradiated, and n = 20 mice received 2 Gy irradiation). Donors are plotted separately in Supplementary Figure 4A. The graphical display in Supplementary Figure 4B shows the data fit by independent regression lines for irradiated and non-irradiated mice. Akaike’s Information Criterion (AICc) test in GraphPad was used to estimate the probability (99.92%) that the irradiated and non-irradiated mouse data was better fit by two independent curves, than a single curve, suggesting that BMSC behavior was statistically different in irradiated and non-irradiated mice