Fig. 1

Notch1 level is elevated in the livers of FXR-KO mice and inversely correlate with FXR level in the DDC-treated mouse livers. a Expression of NICD1 and Notch1 was elevated in liver tumor samples from 12-month-old FXR-KO mice, normalized to LaminB or GAPDH. b Representative photomicrographs shown expression of FXR and Notch1 in 12-month-old WT and FXR-KO mouse liver tissues as assessed by an IF assay. Scale bars: 100 μm. c Expression of FXR and Notch1 mRNAs in the livers of WT and DDC-treated mice. d Expression of FXR and Notch1 in WT and DDC-treated mice was examined by western blotting, normalized to GAPDH. e The liver of DDC-treated mouse samples was co-stained with anti-FXR and anti-Notch1 antibodies, then counterstained with DAPI for confocal microscopy. Scale bars: 10 μm. Data were presented as mean ± SEM of three independent experiments. *p < 0.05