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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Farnesoid X receptor via Notch1 directs asymmetric cell division of Sox9+ cells to prevent the development of liver cancer in a mouse model

Fig. 5

FXR binds to FXRE in Notch1 promoter region and suppresses Notch1 transcriptional activity. a Potential FXRE/DR7 in the Notch1 promoter region was predicted that the site of Notch1 was indicated by red letter, and the FXRE/DR7 was underlined. b pGL3-Notch1 FXRE-wt and pGL3-Notch1 FXRE-mut were separately co-transfected with the Renilla luciferase expression vector pRL-TK into HepG2 cells. After 6 h of incubation, the cells were treated with DMSO or GW4064 (10 μM) for 36 h. c The sequences of the DR7 probe and mutant DR7 probe were shown. d EMSA analysis of the binding of FXR proteins to the DR7 was performed. The position of the up-shifted FXR/RXR a complex was indicated. e ChIP assays were performed using chromatin isolated from GW4064-treated HepG2 cells. Data were means of three separated experiments ± SEM, *p < 0.05

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