Fig. 6

FXR activation inhibits Notch1 and promotes Sox9⁺ cells asymmetric division. a Serum ALT (left) and AST (right) activities were determined. b H&E staining of liver sections from WT and FXR-KO mice. Scale bars: 100 μm. c Liver tissues were subjected to quantitative real-time PCR assay for determination of SHP (left) and Notch1 (right) mRNA levels. d Liver tissues were applied to Western blotting analysis of NICD1, Notch1 and Numb protein levels, normalized to LaminB or GAPDH. e, f Representative images e and quantification f the asymmetric division frequency of Sox9⁺ cells in DDC fed WT and FXR-KO mice with either (DDC/control) or (DDC/GW4064) treatment. Scale bars: 10 μm. g A model for the regulation of asymmetric division by FXR-Notch1. Data represented the mean ± SEM (N = 4–6). Statistical significance of differences between each treatment and control group (*p < 0.05; **p < 0.01) were determined