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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: MSC-derived small extracellular vesicles overexpressing miR-20a promoted the osteointegration of porous titanium alloy by enhancing osteogenesis via targeting BAMBI

Fig. 1

Selection of miR-20a and characterization of hBM-MSC-derived sEVs. a The selection of miR-20a among the five candidates after treated with OM. b The morphology of sEV and sEV-20a was analyzed by TEM. Scale bar = 100 nm. c The diameter of sEV and sEV-20a was measured by NTA. d The uptake of sEV by hBM-MSCs detected by fluorescence staining. Green, red and blue represent the cytoskeleton, sEV and nucleus respectively. Scale bar = 100 μm. e Western-blot was applied for the detection of the specific surface markers (Alix, Tsg101, CD9) of sEV and sEV-20a. Calnexin was used as a negative marker and GAPDH was applied for the internal reference. hBM-MSC group was used for control. f The relative expression of miR-20a in hBM-MSC overexpressing miR-20a. hBM-MSC group was used for control. g The relative gene expression of miR-20a in hBM-MSC-derived sEVs overexpressing miR-20a. sEV group was used for control (*p < 0.05)

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