Fig. 2

Effect of upregulated CD157 expression on BMSCs and VSC4.1 motor neurons. a Representative immunofluorescence images of CD157 protein. BMSCs were pre-stained using CFSE. Normal group, cells without any treatment; OGD group, VSC4.1 motor neurons were treated with oxygen and glucose deprivation for 8 h before co-cultured with BMSCs. The purple arrows indicate VSC4.1 motor Scale bars, 100 μm. b, c CD157 expression quantification (fold of normal) of VSC4.1 motor neurons (b) and BMSCs (c). Image J was used to measure IOD. n.s., non-significant, **p < 0.01. d Transfection effects of mock, Bst-1⁺, and Bst-1⁻ adenovirus vectors, alphabetically. All vectors were recombined with the GFP gene. Scale bar, 100 μm. e Western blotting of CD157 protein expression of transfected BMSCs