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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: CD157 in bone marrow mesenchymal stem cells mediates mitochondrial production and transfer to improve neuronal apoptosis and functional recovery after spinal cord injury

Fig. 3

CD157 overexpression in BMSCs enhances extracellular mitochondria production and transfer. a FACS results of extracellular mitochondria from various transfected BMSCs. Control, BMSCs without pre-staining. b Extracellular mitochondria particle ratio detected by FACS (n = 4). *p < 0.05, **p < 0.01. c Extracellular mitochondria particle quantification detected by Varioskan Flash (n = 5). *p < 0.05, ***p < 0.001. d Representative confocal images of internalized mitochondria of VSC4.1 motor neurons. All VSC4.1 motor neurons were subjected to OGD treatment before direct co-culture with BMSCs. Transfected BMSCs were marked using GFP. The white arrows indicate VSC4.1 motor neurons. Scale bars, 100 μm. f Representative confocal images of internalized mitochondria of VSC4.1 motor neurons. OGD VSC4.1 motor neurons co-cultured with BMSCs in transwell system. VSC4.1 motor neurons were pre-stained using CFSE. Scale bars, 100 μm. e, g Mitochondria internalization quantification of VSC4.1 motor neurons (fold of Mock group). c Direct co-culture and e transwell co-culture. Image J was used to measure IOD. *p < 0.05, ***p < 0.001, ****p < 0.0001

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