Fig. 3From: CD157 in bone marrow mesenchymal stem cells mediates mitochondrial production and transfer to improve neuronal apoptosis and functional recovery after spinal cord injuryCD157 overexpression in BMSCs enhances extracellular mitochondria production and transfer. a FACS results of extracellular mitochondria from various transfected BMSCs. Control, BMSCs without pre-staining. b Extracellular mitochondria particle ratio detected by FACS (n = 4). *p < 0.05, **p < 0.01. c Extracellular mitochondria particle quantification detected by Varioskan Flash (n = 5). *p < 0.05, ***p < 0.001. d Representative confocal images of internalized mitochondria of VSC4.1 motor neurons. All VSC4.1 motor neurons were subjected to OGD treatment before direct co-culture with BMSCs. Transfected BMSCs were marked using GFP. The white arrows indicate VSC4.1 motor neurons. Scale bars, 100 μm. f Representative confocal images of internalized mitochondria of VSC4.1 motor neurons. OGD VSC4.1 motor neurons co-cultured with BMSCs in transwell system. VSC4.1 motor neurons were pre-stained using CFSE. Scale bars, 100 μm. e, g Mitochondria internalization quantification of VSC4.1 motor neurons (fold of Mock group). c Direct co-culture and e transwell co-culture. Image J was used to measure IOD. *p < 0.05, ***p < 0.001, ****p < 0.0001Back to article page