Fig. 4

CD157 overexpression in BMSCs improves the injured VSC4.1 motor neurons in vitro. a Representative images of recovered VSC4.1 motor neurons. The four groups are in alphabetical order: OGD group, Mock group, Over group, and shRNA group. The black arrows show the axons. Scale bar, 50 μm. b Statistics for the number of neurites of each recovered VSC4.1 motor neurons. n.s., non-significant. c Statistics for the length of neurites of each recovered VSC4.1 motor neurons (fold of OGD group). *p < 0.05, **p < 0.01. d–f Respectively, representative immunofluorescence images of Grp 78 protein, NF-κB protein, and Bcl-xl protein of VSC4.1 motor neurons. GFP marked BMSCs. The white arrows show VSC4.1 motor neurons. Scale bars, 100 μm. g Representative images of TUNEL staining of VSC4.1 cells. Scale bar, 200 μm. h–j Respectively, quantification of Grp 78, NF-κB, and Bcl-xl of VSC4.1 motor neurons (fold of OGD group). n.s., non-significant, ****p < 0.0001 vs OGD group. ^#p < 0.05, ^##p < 0.01, ^####p < 0.0001 vs Mock group. k Statistics of TUNEL positive rate of VSC4.1 cells. **p < 0.01, ****p < 0.0001 vs OGD group. ^#p < 0.05, ^##p < 0.01 vs Mock group