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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: The preconditioning of lithium promotes mesenchymal stem cell-based therapy for the degenerated intervertebral disc via upregulating cellular ROS

Fig. 8

Prevention of ROS generation attenuated the effects of LiCl preconditioning to ADSC. a Relative cell number of ADSC microsphere. b The ECM content of ADSC microspheres determined using Alcian Blue staining, scale bar = 50 μm. c The immunofluorescent staining of aggrecan in ADSC microspheres, scale bar = 50 μm. d Quantitative analyses of aggrecan content. e, f ERK1/2 and pERK1/2 expression of preconditioned ADSC with or without APO treatment. g, h the mRNA expression of MMP13 and ADAMTS5 of preconditioned ADSC with or without APO treatment. Error bars depict Mean ± SD. (***P < 0.001, **P < 0.01, *P < 0.05)

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Stem Cell Research & Therapy

ISSN: 1757-6512