Fig. 4

High Pi promotes the translocation of HMGB1 from the nucleus to the cytosol in aortic calcification. CKD mice were placed on a high-phosphate diet for 12 weeks with either PBS or exosome treatment. a Serum concentrations of HMGB1 were measured by ELISA. b Western blot analysis of SIRT6 in the aortas from sham and CKD mice. c Quantification of SIRT6 expression relative to that of β-actin. n = 6 per group. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, compared with sham group. #p < 0.05 compared with the PBS group. d Immunofluorescence detection of HMGB1 (red) and SIRT6 (green). Nuclei were counterstained with DAPI (blue). Scale bar = 100 μm