Fig. 6

Expression of genes involved in osteogenesis increases when SIRT6 is downregulated. Chronic kidney disease (CKD) of NC-shRNA or SIRT6-shRNA lentivirus injection mice were placed on a high-phosphate diet for 12 weeks with either PBS or exosome treatment. a Serum concentrations of HMGB1 were measured by ELISA. b Western blot analysis of osteopontin, Runx2, and Msx2 in the aortas from sham-operated and CKD mice. Quantification of osteopontin (c), Runx2 (d), and Msx2 (e) expression relative to that of β-actin. n = 6 per group. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, compared with the sham-operated group. ^#p < 0.05 compared with the PBS group in NC-shRNA or Sirt6-shRNA lentivirus injected mice