Fig. 10

N-terminus of TRPC7 (TRPC7-N) partially diminished Ca²⁺ permeability of TRPC7 and decreased the frequency of AP in mESC-CMs. a Ca²⁺ imaging of HEK-293-FT cells transfected with different plasmids encoding TRPC7, TRPC7+TRPC7-N, and TRPC7-N respectively, empty plasmid was used as a negative control. Curves showed the average fluorescence intensity calculated from each group. The protocol used to elicit the Ca²⁺ entry via TRPC7 was shown on the top of the chart. b A bar chart showing accumulated intensity calculated from the area under each curve in a from 240 to 600 min. Co-expression of TRPC7-N with TRPC7 could decrease the Ca²⁺ entry mediated by TRPC7. Data were presented as mean ± SEM (n = 100 cells). ns, not significant; ***P < 0.001. Representative APs of mESC-CMs recorded from c overexpression control (BFP only) and d TRPC7-N overexpression groups. Bar charts showing e the frequency, f the DDR, g the amplitude, h APD₅₀, and I the MDP of APs from c and d. Overexpression of TRPC7-N decreased the frequency and DDR without affecting the amplitude, APD₅₀, and MDP of APs. Data were presented as mean ± SEM (n = 17–18 cells; cells were from 5 independent batches of differentiation). *P < 0.05, **P < 0.01 vs control