Fig. 3

Knockdown of TRPC7 decreased while overexpression increased the frequency of localized calcium releases (LCRs) in mESC-CMs. Representative LCRs of mESC-CMs recorded from a knockdown control (shRNA-luciferase) and b TRPC7 knockdown (shRNA-458) groups. Bar charts showing c the frequency, d the amplitude, e the full width at half-maximum (FWHM), and f the full duration at half-maximum (FDHM) of LCRs from a and b. Knockdown of TRPC7 decreased the frequency of LCRs without affecting other parameters. Representative LCRs of mESC-CMs recorded from g overexpression control (BFP only) and h TRPC7 overexpression groups. Bar charts showing i the frequency, j the amplitude, k the FWHM, and l the FDHM of LCRs from g and h. Overexpression of TRPC7 increased the frequency of LCRs without affecting other parameters. Data were presented as mean ± SEM (n = 10–11 cells; cells were from 5 independent batches of differentiation). *P < 0.05 vs corresponding controls