Fig. 5
From: TRPC7 regulates the electrophysiological functions of embryonic stem cell-derived cardiomyocytes
Knockdown of TRPC7 decreased the activity of RyR2 and SERCA without affecting the activity of IP3R in mESC-CMs. Representative CaTs recorded from the control group a before and b after applying ryanodine. Representative CaTs recorded from TRPC7 knockdown group c before and d after applying ryanodine. e, f Bar charts showing the normalized frequency and the Vmax-upstroke calculated from a–d. The decrease of the frequency and the Vmax-upstroke caused by ryanodine was less in TRPC7 knockdown group. Representative CaTs recorded from the control group g before and h after applying thapsigargin (TG). Representative CaTs recorded from TRPC7 knockdown group i before and j after applying TG. k, l Bar charts showing the normalized frequency and Vmax-decay calculated from g–j. The decrease of the frequency and the Vmax-decay and amplitude caused by TG was less in TRPC7 knockdown group. Representative CaTs recorded from the control group m before and n after applying 2-aminoethoxydipheylborate (2-APB). Representative CaTs recorded from TRPC7 knockdown group o before and p after applying 2-APB. q, r Bar charts showing the normalized frequency and the Vmax-upstroke calculated from m–p. The decrease of the frequency and the Vmax-upstroke caused by 2-APB was similar between control and TRPC7 knockdown groups. Data were presented as mean ± SEM (n = 10 cells; cells were from 3 independent batches of differentiation). *P < 0.05, **P < 0.01 vs control