Fig. 4From: Cyclosporine A and IFNγ licencing enhances human mesenchymal stromal cell potency in a humanised mouse model of acute graft versus host diseaseEffects of CsA on IFNγ signalling pathway proteins SOCS1 and pSTAT1. Protein levels of SOCS1, pSTAT1 and total STAT1 were measured by western blot in MSC stimulated with/out IFNγ (50 ng/ml) for at least 24 h followed by exposure to CsA (1000 ng/ml) for 1 h, 6 h or 24 h for the remainder of a 48 h window. All cells were harvested at a 48 h time-point. β-Actin was included as a loading control. Densitometry of n = 3 studies is shown for SOCS1 (b), pSTAT1 (c) and total STAT1 (d). Statistical analysis was carried out using unpaired Student’s t test where * < 0.05 and ** < 0.01Back to article page