Fig. 5
Prestimulation with CsA enhances the potency of IFNγ licenced MSC in a humanised mouse model of acute GvHD. A humanised mouse model of aGvHD was established by administering 8 × 105 human PBMC/gram (or PBS as a control) to irradiated (2.4 Gy) NSG mice on day 0. 6.4 × 104/g MSC, IFNγ (50 ng/ml 24 h) licenced MSC [MSCγ], MSC cultured with CsA (1000 ng/ml) for 24 h [MSC CsA] or MSC stimulated with IFNγ (50 ng/ml) for 24 h followed by CsA (1000 ng/ml) for 24 h [MSCγ CsA] were delivered intravenously on day 6. Mice were monitored for aGvHD development every 2 days until day 9 and then every day for the duration of the experiment (a). Survival curve (b). n = 9 mice per group. Statistical analysis was carried out using a Mantel-Cox test for the survival curve where * < 0.05, ** < 0.01 and *** < 0.001. * with no bar are in comparison to the PBMC group