Fig. 4From: Hypoxic preconditioning induces epigenetic changes and modifies swine mesenchymal stem cell angiogenesis and senescence in experimental atherosclerotic renal artery stenosisGlobal epigenetic alterations in ARAS (N = 4) compared to healthy MSCs (N = 5) under normoxic and hypoxic conditions demonstrated by: Dot-blotting showing MSC DNA hydroxymethylation measuring genomic 5-hydroxymethyl-cytosine (5hmC). a Western blotting of histone-3 protein measuring genomic trimethylation on lysine-4 (H3K4me3), 9 (H3K9me3), and 27 (H3K27me3) residues. b Genomic 5hmC was reduced during HPC, while H3K4me3 and H3K27me3 marks in ARAS were elevated as compared to healthy controls at baseline and during HPC. No differences in H3K9 levels. HN healthy normoxia, HH healthy hypoxia, ARAS-N ARAS normoxia, ARAS-H ARAS hypoxia. *p < 0.05 ARAS hypoxia vs ARAS normoxia. #p < 0.05 healthy hypoxia vs healthy normoxia. ‡p < 0.05 ARAS normoxia vs healthy normoxia. †p < 0.05 ARAS hypoxia vs healthy hypoxiaBack to article page