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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Andrographolide promotes proliferative and osteogenic potentials of human placenta-derived mesenchymal stem cells through the activation of Wnt/β-catenin signaling

Fig. 2

Effect of AP on cell viability and proliferation of PL-hMSCs. a Cell viability of PL-hMSCs in growth medium with various concentrations of AP (0.1–50 μM) at 24, 48, and 72 h. b Cell proliferation of PL-hMSC in growth medium with various concentrations of AP (1, 2.5, 5, 10 μM) at 24, 48, 72, 96, and 120 h. Data are presented as the means of independent experiments (n = 4). *P < 0.05, ***P < 0.01, and ***P < 0.001 significant increases compared to the control group at the same time point, #P < 0.05, significant decreases compared to the control group at the same time point. c Cell morphology of PL-hMSCs after treatment with 2.5 μM AP for 5 days. d The % of cell surface markers expressions in PL-hMSCs after treatment with 2.5 μM AP for 5 days performed by flow cytometry (positive for CD73, CD90, and CD105; negative for CD34 and CD45). Data are expressed as mean ± SEM; n = 3

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