Table 1 Sequences of primers for quantitative RT-PCR analyses
Gene | Sequence (5′ to 3′) | Length of product (bp) |
|---|---|---|
Runx2 | F-5′-CCTCGGAGAGGTACCAGATG-3′ R-5′-TTCCCGAGGTCCATCTACTG-3′ | 247 |
OSX | F-5′-GCCAGAAGCTGTGAAACCTC-3′ R-5′-GCTGCAAGCTCTCCATAACC-3′ | 161 |
ALP | F-5′-CCTTGCTCACTCACTCACTCC-3′ R-5′-TTTTTTTTGCCGTTCCAAAC-3′ | 182 |
Osteocalcin | F-5′-GTGCAGAGTCCAGCAAAGGT-3′ R-5′-TCAGCCAACTCGTCACAGTC-3′ | 152 |
Col1a1 | F-5′-AGGGCCAAGACGAAGACATCCC-3′ R-5′-TGTCGCAGACGCAGATCCG-3′ | 108 |
OPG | F-5′-AACGCCAACACAGCTCACAAGAAC-3′ R-5′-TGCTCGAAGGTGAGGTTAGCATGT-3′ | 160 |
RANKL | F-5′-ATCGTTGGATCACAGCACATC-3′ R-5′-AGACTCACTTTATGGGAACCAGA-3′ | 152 |
C-myc | F-5′-ATGGCCCATTACAAAGCCG-3′ R-5′-TTTCTGGAGTAGCAGCTCCTAA-3′ | 175 |
AXIN2 | F-5′-CCTGGCTCCAGAAGATCACA-3′ R-5′-AGCATCCTCCGGTATGGAAT-3′ | 120 |
Survivin | F-5′-TGAGAACGAGCCAGACTTG-3′ R-5′-TGTTCCTCTATGGGGTCGTCA-3′ | 87 |
Cyclin D1 | F-5′-GATCAAGTGTGACCCGGACTG-3′ R-5′-CCTTGGGGTCCATGTTCTGC-3′ | 101 |
GAPDH | F-5′-GAGTCAACGGATTTGGTCGT-3′ R-5′-TTGATTTTGGAGGGATCTCG-3′ | 238 |