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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Classical isoforms of protein kinase C (PKC) and Akt regulate the osteogenic differentiation of human dental follicle cells via both β-catenin and NF-κB

Fig. 5

Regulation of NF-κB pathway after inhibition of PKC and Akt. DFCs were cultivated in control medium (DMEM), osteogenic differentiation medium (ODM), or BMP2 containing differentiation medium with or without simultaneous treatment with 100 nM classical PKC inhibitor Gö6976 for 7 days (a, c, e, g, i) or 200 nM Akt inhibitor MK2206 for 8 days (b, d, f, h, j). Expression of NF-κB (p65 subunit, a, b), P-NF-κB (p65 subunit, Ser536, c, d), IκBα (e, f), IKKα (g, h), and IKKβ (I, J) was determined by Western blot analysis. Phospho-ratios (c, d) were calculated as ratios between phosphorylated and total NF-κB. Bar charts show means + SD (n = 3). Student’s t test was performed to determine statistically significant differences between the control and treatment group for each medium. *p < 0.05, **p < 0.01, ***p < 0.001

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