Fig. 6

Activation of PKC and Akt by Wnt5a and PTHrP. Undifferentiated DFCs were transfected with specific siRNA against WNT5A (Wnt5a, a, b) or PTHLH (PTHrP, c–e) or control siRNA for 3 days. Expression of P-PKC (phosphorylation sites according to Ser660 on PKC βII, a, c), P-Akt (Ser473, b, d), and NF-κB (e) was determined by Western blot analysis. Bar charts show means + SD (n = 3). Student’s t test was performed to determine statistically significant differences in comparison to control siRNA. *p < 0.05, **p < 0.01, ***p < 0.001