Fig. 5

Mature cell characteristics. a Mature hESC-RPE cells grown on PET membranes at day 28. b Fluorescent detection of F-actin (phalloidin) on mature hESC-RPE monolayers (left) with DIC photomicroscopy (right). Scale = 100 μm. c Confocal Z-stack analysis of F-actin (Phalloidin) and MCT-1 in mature hESC-RPE monolayers. Scale = 50 μm. d Uptake of fluorescent beads coated with VXF or BSA (control) demonstrate receptor-mediated phagocytosis. Scale = 400 μm. Z-stack confocal microscopy shows internalization of beads, shown below cell membranes. Phalloidin staining of F-actin (green) shows apical membrane of hESC-RPE cells. Scale = 15 μm). e Measurement of transepithelial electrical resistance (TEER) over 6 weeks (n = 4). f Directional secretion of VEGF and PEDF from mature hESC-RPE cells (n = 3)