Fig. 3From: Curcumin-primed human BMSC-derived extracellular vesicles reverse IL-1β-induced catabolic responses of OA chondrocytes by upregulating miR-126-3pCharacterization of control EVs and Cur-EVs. a Representative western blot image showing bands of standard surface markers (CD9, CD63, CD81) of control EV- and Cur-EV lysates. b, c Morphology of control EVs and Cur-EVs was monitored by TEM; scale bar, 100 nm. d, e Particle size distribution of control EVs and Cur-EVs was measured by NTA. f, g Quantitative comparison between control EVs and Cur-EVs in count and size measured by NTA; n = 3. h Absorbance at 420 nm of control EVs and Cur-EVs was determined with by spectrophotometry indicating presence of curcumin in Cur-EVs; n = 3. i Cell nuclei were stained with DAPI (blue) and chondrocytes were stained with Phalloidin (green) to visualize the structure of the cytoskeleton. PKH26-labeled control EVs (red) and Cur-EVs (red) were internalized by chondrocytes and visualized with fluorescent microscopyBack to article page