Fig. 5
Effect of control EVs and Cur-EVs on gene expression of IL-1β-induced OA-CH. After 24 h treatment with IL-1β and/or Cur-mediumultra, free curcumin (10 μM), control EVs and Cur-EVs, expression levels of anabolic genes (a–d BCL2, ACAN, SOX9, COL2A1) and catabolic genes (e–h IL1B, IL6, MMP13, COL10A1) were determined with real-time RT-PCR analysis; n = 4. All values represent the mean ± standard deviation. Difference to no treatment controls (OA-CH): *p < 0.05; **p < 0.01;***p < 0.001; ****p < 0.0001, #difference between groups: #p < 0.05; ##p < 0.01; ###p < 0.001; ####p < 0.0001; 1-way ANOVA with Newman-Keuls multiple comparison test