Table 1 Primer sequences for qPCR

ACAN

Fwd: 5′-CTATACCCCAGTGGGCACAT-3′

Rev:5′-GGCACTTCAGTTGCAGAAGG − 3′

BCL2

Fwd: 5′-ATGTGTGTGGAGAGCGTCAA-3′

Rev: 5′-ACAGTTCCACAAAGGCATCC-3′

COL2A1

Fwd: 5′-CCAGATGACCTTCCTACGCC-3′

Rev: 5′-TTCAGGGCAGTGTACGTGAAC-3′

SOX9

Fwd:5′-GTACCCGCACTTGCACAAC-3′

Rev: 5′-TCTCGCTCTCGTTCAGAAGTC-3′

IL6

Fwd: 5′-CAATGAGGAGACTTGCCTGG-3′

Rev: 5′-GCACAGCTCTGGCTTGTTCC-3′

COL10A1

Fwd:5′-CAC GTT TGG GTA GGC CTG TA-3′

Rev: 5′-TCT GTG AGC TCC ATG ATT GC-3′

IL-1beta

Fwd: 5′-TAAGCCCACTCTACAGCTGG-3′

Rev: 5′-GAGAGGTGCTGATGTACCAG-3′

MMP13

Fwd:5′-GACTGGTAATGGCATCAAGGGA-3′

Rev: 5′-CACCGGCAAAAGCCACTTTA-3′

TBP

Fwd:5′-TTG TAC CGCAGCTGCAAA AT-3′

Rev: 5′-TAT ATT CGG CGT TTC GGG CA-3′

GAPDH

Fwd: 5′-CTGACTTCAACAGCGACACC-3′

Rev: 5′-CCCTGTTGCTGTAGCCAA AT-3′