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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Melatonin induces the rejuvenation of long-term ex vivo expanded periodontal ligament stem cells by modulating the autophagic process

Fig. 3

MLT rescues cells from long-term ex vivo passaging-induced senescence. (MLT-treated P2, P7, and P15 cells were termed P2 + MLT, P7 + MLT, and P15 + MLT, respectively). *p < 0.05, **p < 0.01, and ****p < 0.0001 represent significant differences between the indicated columns. a Representative images of SA-β-gal expression in P2, P7, and P15 cells (immunocytochemical staining, positively stained cells were dyed in blue; scale bar = 100 μm). b Percentage of SA-β-gal-positive cells among all cells and quantitative analysis. c Cell senescence-related protein expression in P2, P7, and P15 cells as determined by Western blot assay. d Quantitative analysis of p53, p21, p16, and γ-H2AX expression in P2, P7, and P15 cells

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