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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Melatonin induces the rejuvenation of long-term ex vivo expanded periodontal ligament stem cells by modulating the autophagic process

Fig. 8

Downregulation of autophagy via MT/PI3K/AKT/mTOR signaling deteriorates senescence in cells undergoing long-term ex vivo passaging. (CON: P15 cells without any treatment; MLT: MLT-treated P15 cells; MLT + LUZ: MLT plus LUZ-treated P15 cells; MLT + SC79: MLT plus SC79-treated P15 cells; MLT + MHY1485: MLT plus MHY1485-treated P15 cells). *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 represent significant differences between the indicated columns, while NS represents no significant difference. a Representative images of SA-β-gal expression in P15 cells with or without MLT/LUZ/SC79/MHY1485 treatment (immunocytochemical staining, positively stained cells were dyed in blue; scale bar = 100 μm). b Percentage of SA-β-gal-positive cells among all cells and quantitative analysis. c Cell senescence-related protein expression in P15 cells with or without MLT/LUZ/SC79/MHY1485 treatment as determined by Western blot assay. d Quantitative analysis of p53, p21, p16, and γ-H2AX expression in P15 cells with or without MLT/LUZ/SC79/MHY1485 treatment

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Stem Cell Research & Therapy

ISSN: 1757-6512